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p jak2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p jak2
    P Jak2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+p+jak2/Jak2+XP+Rabbit+mAb/pm41910173-335-34-36
    Average 96 stars, based on 1265 article reviews
    p jak2 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Angiotensin II Receptor-Associated Protein (AGTRAP) Enhances Glioma Cell Survival Through the IL-6/JAK2/STAT3 Pathway and Correlates With an Immunosuppressive Microenvironment.
    Article Snippet: .. Membranes were incubated overnight at 4°C with primary antibodies, including anti- AGTRAP (GeneTex, GTX64875, 1:1000), anti- CDK2 (Beyotime, AF1063, 1:500), anti- Bcl2 (abcam, ab182858, 1:2000), anti- Bax (abcam, ab32503, 1:1000), anti- cleaved- caspase- 3 (abcam, ab2302, 1:1000), anti- IL- 6 (abcam, ab214429, 1:1000), anti- p- JAK2 (CST, 8082, 1:1000), anti- JAK2 (CST, 3230, 1:1000), anti- pSTAT3 (CST, 9145, 1:1000), anti- STAT3 (CST, 9139, 1:1000), and anti- β- actin (abcam, ab8226, 1:2000). .. The following day, membranes were incubated with secondary antibodies and developed using an ECL detection system (Biorad).

    Article Title: Endogenous Ceramide 24:1 Constrains Th17-Driven Neutrophilic Inflammation by Antagonizing EP2 Signaling.
    Article Snippet: Advanced Science, 2026 om m ons L icense nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202520695 by IN A SP - N E PA L , W iley O nline L ibrary on [15/03/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C For WB analysis, the protein lysates were separated via SDS ‒PAGE, transferred to PVDF membranes, and incubated overnight at 4◦C with the following primary antibodies: antiSmpd1 (1:1000, ABclonal, #39147-1), anti-Cers2 (1:1000, Abcam, #ab31545), anti-Sptlc2 (1:1000, Abcam, #ab229330), anti-Ror γt (1:1000, Proteintech, #29910-1-AP), anti-Jak2 (1:1000, CST, #3230), anti-p-Jak2 (1:1000, CST, #8082S), anti-Stat3 (1:1000, CST, #4904T), anti-p-Stat3 (1:1000, CST, #9145T), anti-EP2 (1:1000, Abcam, #ab167171), anti-EP4 (1:1000, Proteintech, #24895-1-AP), anti-CXCR4 (1:1000, Abcam, #ab181020), anti-CREB (1:1000, CST, #9197), anti-p-CREB (1:1000, CST, #9198), and anti- βActin (1:1000, Proteintech, #20536-1-AP). ..

    Article Title: Angiotensin II Receptor‐Associated Protein ( AGTRAP ) Enhances Glioma Cell Survival Through the IL ‐6/ JAK2 / STAT3 Pathway and Correlates With an Immunosuppressive Microenvironment
    Article Snippet: .. Membranes were incubated overnight at 4°C with primary antibodies, including anti‐AGTRAP (GeneTex, GTX64875, 1:1000), anti‐CDK2 (Beyotime, AF1063, 1:500), anti‐Bcl2 (abcam, ab182858, 1:2000), anti‐Bax (abcam, ab32503, 1:1000), anti‐cleaved‐caspase‐3 (abcam, ab2302, 1:1000), anti‐IL‐6 (abcam, ab214429, 1:1000), anti‐p‐JAK2 (CST, 8082, 1:1000), anti‐JAK2 (CST, 3230, 1:1000), anti‐p‐STAT3 (CST, 9145, 1:1000), anti‐STAT3 (CST, 9139, 1:1000), and anti‐β‐actin (abcam, ab8226, 1:2000). .. The following day, membranes were incubated with secondary antibodies and developed using an ECL detection system (Biorad).

    Western Blot:

    Article Title: Endogenous Ceramide 24:1 Constrains Th17-Driven Neutrophilic Inflammation by Antagonizing EP2 Signaling.
    Article Snippet: Advanced Science, 2026 om m ons L icense nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202520695 by IN A SP - N E PA L , W iley O nline L ibrary on [15/03/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C For WB analysis, the protein lysates were separated via SDS ‒PAGE, transferred to PVDF membranes, and incubated overnight at 4◦C with the following primary antibodies: antiSmpd1 (1:1000, ABclonal, #39147-1), anti-Cers2 (1:1000, Abcam, #ab31545), anti-Sptlc2 (1:1000, Abcam, #ab229330), anti-Ror γt (1:1000, Proteintech, #29910-1-AP), anti-Jak2 (1:1000, CST, #3230), anti-p-Jak2 (1:1000, CST, #8082S), anti-Stat3 (1:1000, CST, #4904T), anti-p-Stat3 (1:1000, CST, #9145T), anti-EP2 (1:1000, Abcam, #ab167171), anti-EP4 (1:1000, Proteintech, #24895-1-AP), anti-CXCR4 (1:1000, Abcam, #ab181020), anti-CREB (1:1000, CST, #9197), anti-p-CREB (1:1000, CST, #9198), and anti- βActin (1:1000, Proteintech, #20536-1-AP). ..

    Immunohistochemical staining:

    Article Title: α-terpineol induces apoptosis in melanoma cells and its underlying mechanism.
    Article Snippet: .. Immunohistochemical analysis involved staining tumor sections with anti-p-JAK2 and anti-p-STAT3 antibodies to assess cell proliferation, and nuclear proliferative activity was evaluated using rabbit anti-human Ki-67 monoclonal antibodies (1:400, Cell Signaling Technology, Boston, USA). .. Apoptosis was assessed using a TUNEL reaction mixture composed of 50 μL Solution 1 and 450 μL Solution 2 from the In Situ Cell Death Detection Kit POD (Roche).

    Staining:

    Article Title: α-terpineol induces apoptosis in melanoma cells and its underlying mechanism.
    Article Snippet: .. Immunohistochemical analysis involved staining tumor sections with anti-p-JAK2 and anti-p-STAT3 antibodies to assess cell proliferation, and nuclear proliferative activity was evaluated using rabbit anti-human Ki-67 monoclonal antibodies (1:400, Cell Signaling Technology, Boston, USA). .. Apoptosis was assessed using a TUNEL reaction mixture composed of 50 μL Solution 1 and 450 μL Solution 2 from the In Situ Cell Death Detection Kit POD (Roche).

    Activity Assay:

    Article Title: α-terpineol induces apoptosis in melanoma cells and its underlying mechanism.
    Article Snippet: .. Immunohistochemical analysis involved staining tumor sections with anti-p-JAK2 and anti-p-STAT3 antibodies to assess cell proliferation, and nuclear proliferative activity was evaluated using rabbit anti-human Ki-67 monoclonal antibodies (1:400, Cell Signaling Technology, Boston, USA). .. Apoptosis was assessed using a TUNEL reaction mixture composed of 50 μL Solution 1 and 450 μL Solution 2 from the In Situ Cell Death Detection Kit POD (Roche).

    Bioprocessing:

    Article Title: α-terpineol induces apoptosis in melanoma cells and its underlying mechanism.
    Article Snippet: .. Immunohistochemical analysis involved staining tumor sections with anti-p-JAK2 and anti-p-STAT3 antibodies to assess cell proliferation, and nuclear proliferative activity was evaluated using rabbit anti-human Ki-67 monoclonal antibodies (1:400, Cell Signaling Technology, Boston, USA). .. Apoptosis was assessed using a TUNEL reaction mixture composed of 50 μL Solution 1 and 450 μL Solution 2 from the In Situ Cell Death Detection Kit POD (Roche).



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    Naringin inhibits <t>JAK2/STAT3</t> signaling and restores tight junction proteins in dextran sulfate sodium-induced colitis. (A) Representative western blotting images of p-JAK2, JAK2, p-STAT3, STAT3, IL-6, occludin, ZO-1 and β-actin expression in colon tissues. Densitometric semi-quantification of the relative protein expression levels of (B) p-JAK2/β-actin, (C) JAK2/β-actin, (D) p-JAK2/JAK2, (E) p-STAT3/β-actin, (F) STAT3/β-actin, (G) p-STAT3/STAT3, (H) IL-6/β-actin, (I) occludin/β-actin and (J) ZO-1/β-actin. Data are presented as mean ± SEM. n=3. *P<0.05 and **P<0.01 vs. control group; # P<0.05 and ## P<0.01 vs. normal group. One-way ANOVA followed by Tukey's post-hoc test was applied. N, normal group; C, control group; Nar, naringin group; Mes, mesalazine group; p-, phosphorylated; JAK2, Janus kinase 2; STAT3, signal transducer and activator of transcription 3; IL-6, ZO-1, zona occludens-1.
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    Naringin inhibits <t>JAK2/STAT3</t> signaling and restores tight junction proteins in dextran sulfate sodium-induced colitis. (A) Representative western blotting images of p-JAK2, JAK2, p-STAT3, STAT3, IL-6, occludin, ZO-1 and β-actin expression in colon tissues. Densitometric semi-quantification of the relative protein expression levels of (B) p-JAK2/β-actin, (C) JAK2/β-actin, (D) p-JAK2/JAK2, (E) p-STAT3/β-actin, (F) STAT3/β-actin, (G) p-STAT3/STAT3, (H) IL-6/β-actin, (I) occludin/β-actin and (J) ZO-1/β-actin. Data are presented as mean ± SEM. n=3. *P<0.05 and **P<0.01 vs. control group; # P<0.05 and ## P<0.01 vs. normal group. One-way ANOVA followed by Tukey's post-hoc test was applied. N, normal group; C, control group; Nar, naringin group; Mes, mesalazine group; p-, phosphorylated; JAK2, Janus kinase 2; STAT3, signal transducer and activator of transcription 3; IL-6, ZO-1, zona occludens-1.
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    Naringin inhibits <t>JAK2/STAT3</t> signaling and restores tight junction proteins in dextran sulfate sodium-induced colitis. (A) Representative western blotting images of p-JAK2, JAK2, p-STAT3, STAT3, IL-6, occludin, ZO-1 and β-actin expression in colon tissues. Densitometric semi-quantification of the relative protein expression levels of (B) p-JAK2/β-actin, (C) JAK2/β-actin, (D) p-JAK2/JAK2, (E) p-STAT3/β-actin, (F) STAT3/β-actin, (G) p-STAT3/STAT3, (H) IL-6/β-actin, (I) occludin/β-actin and (J) ZO-1/β-actin. Data are presented as mean ± SEM. n=3. *P<0.05 and **P<0.01 vs. control group; # P<0.05 and ## P<0.01 vs. normal group. One-way ANOVA followed by Tukey's post-hoc test was applied. N, normal group; C, control group; Nar, naringin group; Mes, mesalazine group; p-, phosphorylated; JAK2, Janus kinase 2; STAT3, signal transducer and activator of transcription 3; IL-6, ZO-1, zona occludens-1.
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    Naringin inhibits <t>JAK2/STAT3</t> signaling and restores tight junction proteins in dextran sulfate sodium-induced colitis. (A) Representative western blotting images of p-JAK2, JAK2, p-STAT3, STAT3, IL-6, occludin, ZO-1 and β-actin expression in colon tissues. Densitometric semi-quantification of the relative protein expression levels of (B) p-JAK2/β-actin, (C) JAK2/β-actin, (D) p-JAK2/JAK2, (E) p-STAT3/β-actin, (F) STAT3/β-actin, (G) p-STAT3/STAT3, (H) IL-6/β-actin, (I) occludin/β-actin and (J) ZO-1/β-actin. Data are presented as mean ± SEM. n=3. *P<0.05 and **P<0.01 vs. control group; # P<0.05 and ## P<0.01 vs. normal group. One-way ANOVA followed by Tukey's post-hoc test was applied. N, normal group; C, control group; Nar, naringin group; Mes, mesalazine group; p-, phosphorylated; JAK2, Janus kinase 2; STAT3, signal transducer and activator of transcription 3; IL-6, ZO-1, zona occludens-1.
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    Engineered probiotic PG mitigated radiation-induced intestinal injury by regulating the <t>SOCS1/JAK2/STAT3</t> pathway. a Principal Component Analysis (PCA) revealed differences in gene expression levels among groups. b KEGG Enrichment BarPlot for IR vs. IR + PG displayed the number of differential genes in the pathway represented on the horizontal axis, pathway names on the vertical axis, and colors representing KEGG Level 1 classification. c KEGG-enriched bar graph displaying the top 20 KEGG pathways with the smallest P-values. d The results of the KEGG enrichment analysis werw presented as a scatter plot. (e) The heatmap illustrated the relative expression levels of JAK-STAT signaling pathway-related genes in the IR and IR + PG groups. f Western blot analysis demonstrated the protein expression levels of SOCS1, p-JAK2, JAK2, p-STAT3, and STAT3 across groups
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    Image Search Results


    Naringin inhibits JAK2/STAT3 signaling and restores tight junction proteins in dextran sulfate sodium-induced colitis. (A) Representative western blotting images of p-JAK2, JAK2, p-STAT3, STAT3, IL-6, occludin, ZO-1 and β-actin expression in colon tissues. Densitometric semi-quantification of the relative protein expression levels of (B) p-JAK2/β-actin, (C) JAK2/β-actin, (D) p-JAK2/JAK2, (E) p-STAT3/β-actin, (F) STAT3/β-actin, (G) p-STAT3/STAT3, (H) IL-6/β-actin, (I) occludin/β-actin and (J) ZO-1/β-actin. Data are presented as mean ± SEM. n=3. *P<0.05 and **P<0.01 vs. control group; # P<0.05 and ## P<0.01 vs. normal group. One-way ANOVA followed by Tukey's post-hoc test was applied. N, normal group; C, control group; Nar, naringin group; Mes, mesalazine group; p-, phosphorylated; JAK2, Janus kinase 2; STAT3, signal transducer and activator of transcription 3; IL-6, ZO-1, zona occludens-1.

    Journal: Molecular Medicine Reports

    Article Title: Naringin ameliorates intestinal injury in ulcerative colitis model mice by modulating the JAK2/STAT3 signaling pathway

    doi: 10.3892/mmr.2026.13805

    Figure Lengend Snippet: Naringin inhibits JAK2/STAT3 signaling and restores tight junction proteins in dextran sulfate sodium-induced colitis. (A) Representative western blotting images of p-JAK2, JAK2, p-STAT3, STAT3, IL-6, occludin, ZO-1 and β-actin expression in colon tissues. Densitometric semi-quantification of the relative protein expression levels of (B) p-JAK2/β-actin, (C) JAK2/β-actin, (D) p-JAK2/JAK2, (E) p-STAT3/β-actin, (F) STAT3/β-actin, (G) p-STAT3/STAT3, (H) IL-6/β-actin, (I) occludin/β-actin and (J) ZO-1/β-actin. Data are presented as mean ± SEM. n=3. *P<0.05 and **P<0.01 vs. control group; # P<0.05 and ## P<0.01 vs. normal group. One-way ANOVA followed by Tukey's post-hoc test was applied. N, normal group; C, control group; Nar, naringin group; Mes, mesalazine group; p-, phosphorylated; JAK2, Janus kinase 2; STAT3, signal transducer and activator of transcription 3; IL-6, ZO-1, zona occludens-1.

    Article Snippet: Membranes were subsequently incubated separately with primary antibodies for p-JAK2 (1:1,000; cat. no. bs-2485R; BIOSS), JAK2 (1:1,000; cat. no. bs-0908R; BIOSS), p-STAT3 (1:1,000; cat. no. bs-1658R; BIOSS), STAT3 (1:1,000; cat. no. bs-55208R; BIOSS), occludin (1:1,000; cat. no. A2601; ABclonal Biotech Co., Ltd.), ZO-1 (1:1,000; cat. no. A0659; ABclonal Biotech Co., Ltd.) and β-actin (1:1,000; cat. no. ab8227; Abcam), and then with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:5,000; cat. no. ab6721; Abcam).

    Techniques: Western Blot, Expressing, Control

    Naringin suppresses JAK2/STAT3 activation in IL-6-stimulated Caco-2 cells with STAT3 silencing. (A) Western blot analysis of p-JAK2, JAK2, p-STAT3, STAT3, occludin and ZO-1 in Caco-2 cells under indicated treatments. (B) Validation of STAT3 knockdown efficiency: Relative STAT3 expression in cells transfected with siSTAT3 vs. siNC. ## P<0.01 vs. siNC. Densitometric semi-quantification of relative protein expression levels of (C) p-JAK2/β-actin, (D) JAK2/β-actin, (E) p-JAK2/JAK2, (F) p-STAT3/β-actin, (G) STAT3/β-actin, (H) p-STAT3/ STAT3, (I) occludin/β-actin, (J) ZO-1/β-actin. Data are presented as mean ± SEM. n=3. *P<0.05 and **P<0.01 vs. IL-6 group; ## P<0.01 vs. normal group; Δ P<0.05 and ΔΔ P<0.01 IL-6 + Nar group vs. IL-6 + Nar + siSTAT3 group. One-way ANOVA followed by Tukey's post-hoc test was applied. N, normal group; Nar, naringin group; Mes, mesalazine group; p-, phosphorylated; JAK2, Janus kinase 2; STAT3, signal transducer and activator of transcription 3; ZO-1, zona occludens-1; si, small interfering RNA; NC, negative control.

    Journal: Molecular Medicine Reports

    Article Title: Naringin ameliorates intestinal injury in ulcerative colitis model mice by modulating the JAK2/STAT3 signaling pathway

    doi: 10.3892/mmr.2026.13805

    Figure Lengend Snippet: Naringin suppresses JAK2/STAT3 activation in IL-6-stimulated Caco-2 cells with STAT3 silencing. (A) Western blot analysis of p-JAK2, JAK2, p-STAT3, STAT3, occludin and ZO-1 in Caco-2 cells under indicated treatments. (B) Validation of STAT3 knockdown efficiency: Relative STAT3 expression in cells transfected with siSTAT3 vs. siNC. ## P<0.01 vs. siNC. Densitometric semi-quantification of relative protein expression levels of (C) p-JAK2/β-actin, (D) JAK2/β-actin, (E) p-JAK2/JAK2, (F) p-STAT3/β-actin, (G) STAT3/β-actin, (H) p-STAT3/ STAT3, (I) occludin/β-actin, (J) ZO-1/β-actin. Data are presented as mean ± SEM. n=3. *P<0.05 and **P<0.01 vs. IL-6 group; ## P<0.01 vs. normal group; Δ P<0.05 and ΔΔ P<0.01 IL-6 + Nar group vs. IL-6 + Nar + siSTAT3 group. One-way ANOVA followed by Tukey's post-hoc test was applied. N, normal group; Nar, naringin group; Mes, mesalazine group; p-, phosphorylated; JAK2, Janus kinase 2; STAT3, signal transducer and activator of transcription 3; ZO-1, zona occludens-1; si, small interfering RNA; NC, negative control.

    Article Snippet: Membranes were subsequently incubated separately with primary antibodies for p-JAK2 (1:1,000; cat. no. bs-2485R; BIOSS), JAK2 (1:1,000; cat. no. bs-0908R; BIOSS), p-STAT3 (1:1,000; cat. no. bs-1658R; BIOSS), STAT3 (1:1,000; cat. no. bs-55208R; BIOSS), occludin (1:1,000; cat. no. A2601; ABclonal Biotech Co., Ltd.), ZO-1 (1:1,000; cat. no. A0659; ABclonal Biotech Co., Ltd.) and β-actin (1:1,000; cat. no. ab8227; Abcam), and then with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:5,000; cat. no. ab6721; Abcam).

    Techniques: Activation Assay, Western Blot, Biomarker Discovery, Knockdown, Expressing, Transfection, Small Interfering RNA, Negative Control

    Engineered probiotic PG mitigated radiation-induced intestinal injury by regulating the SOCS1/JAK2/STAT3 pathway. a Principal Component Analysis (PCA) revealed differences in gene expression levels among groups. b KEGG Enrichment BarPlot for IR vs. IR + PG displayed the number of differential genes in the pathway represented on the horizontal axis, pathway names on the vertical axis, and colors representing KEGG Level 1 classification. c KEGG-enriched bar graph displaying the top 20 KEGG pathways with the smallest P-values. d The results of the KEGG enrichment analysis werw presented as a scatter plot. (e) The heatmap illustrated the relative expression levels of JAK-STAT signaling pathway-related genes in the IR and IR + PG groups. f Western blot analysis demonstrated the protein expression levels of SOCS1, p-JAK2, JAK2, p-STAT3, and STAT3 across groups

    Journal: Bioresources and Bioprocessing

    Article Title: Propionate-engineered probiotics reduce radiation-induced intestinal damage

    doi: 10.1186/s40643-026-01020-9

    Figure Lengend Snippet: Engineered probiotic PG mitigated radiation-induced intestinal injury by regulating the SOCS1/JAK2/STAT3 pathway. a Principal Component Analysis (PCA) revealed differences in gene expression levels among groups. b KEGG Enrichment BarPlot for IR vs. IR + PG displayed the number of differential genes in the pathway represented on the horizontal axis, pathway names on the vertical axis, and colors representing KEGG Level 1 classification. c KEGG-enriched bar graph displaying the top 20 KEGG pathways with the smallest P-values. d The results of the KEGG enrichment analysis werw presented as a scatter plot. (e) The heatmap illustrated the relative expression levels of JAK-STAT signaling pathway-related genes in the IR and IR + PG groups. f Western blot analysis demonstrated the protein expression levels of SOCS1, p-JAK2, JAK2, p-STAT3, and STAT3 across groups

    Article Snippet: The primary antibodies included anti-SOCS1 (PSH09-68), JAK2 (SY0245), p-JAK2 (SY24-03), STAT3 (SY24-08), p-STAT3 (SZ43-01), and GAPDH (SA30-01), all purchased from HuaBio-Antibodies Co., Ltd (Hangzhou, China).

    Techniques: Gene Expression, Expressing, Western Blot